7QP3

Pseudogymnoascus pannorum M36 protease without the propeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	39.080, 67.490, 130.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.930 - 1.850
R-factor	0.1514
Rwork	0.149
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k90
RMSD bond length	0.010
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.930	1.960
High resolution limit [Å]	1.840	1.840
Number of reflections	30553	4782
<I/σ(I)>	8.93
Completeness [%]	99.7
Redundancy	12.57
CC(1/2)	1.000	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M potassium thiocyanate, 30% polyethylene glycol monomethyl ether 2,000