7QP2

1-deazaguanosine modified-RNA Sarcin Ricin Loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9
Spacegroup name	P 43
Unit cell lengths	29.480, 29.480, 76.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 0.900
R-factor	0.1171
Rwork	0.116
R-free	0.13750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.007
RMSD bond angle	1.282
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	0.920
High resolution limit [Å]	0.900	0.900
Number of reflections	48142	3548
<I/σ(I)>	35.27
Completeness [%]	100.0
Redundancy	20
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.2 M (NH4)2SO4, 50 mM K-MOPS pH 7.0, 10 mM MgCl2, 10 mM MnCl2