7QP1
Crystal structure of metacaspase from candida glabrata with calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 97.370, 97.370, 54.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.990 - 3.000 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f6o |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.099 | 1.000 |
| Number of reflections | 11590 | 2039 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.7 |
| CC(1/2) | 0.999 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.6 | 292 | (NH4)2SO4 0,2 M, 30% (m/v) PEG 4000 |
