7QOT
Factor XI and Plasma Kallikrein apple domain structures reveals different kininogen bound complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.796 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 87.319, 87.319, 629.548 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.530 - 3.240 |
| R-factor | 0.254 |
| Rwork | 0.251 |
| R-free | 0.28360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2F83 4bdx |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.600 | 3.369 |
| High resolution limit [Å] | 3.240 | 3.241 |
| Number of reflections | 19451 | 972 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 94.9 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.994 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 288 | 0.1M Tris pH8, 1.5M AMMONIUM SULFATE |
