Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.190, 88.190, 499.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.999 |
| Rwork | 0.190 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jyi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.558 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.850 | 2.050 |
| High resolution limit [Å] | 1.999 | 1.999 |
| Number of reflections | 132474 | 9279 |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 98.6 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.990 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG-4000, 0.2 M calcium acetate, 0.1 M Tris-HCl, pH 7.5 |
