7QON
Monoclinic triose phosphate isomerase from Fasciola hepatica.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.393, 110.464, 118.143 |
| Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
| Resolution | 51.809 - 1.510 |
| Rwork | 0.165 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jk2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PARROT |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.809 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.059 | 1.457 |
| Number of reflections | 167030 | 8244 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.999 | 0.321 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | 0.2 M Li2SO4, 0.1 M ADA pH 6.5, 30 % v/v PEG 400 |
