7QOA

Structure of CodB, a cytosine transporter in an outward-facing conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	C 2 2 21
Unit cell lengths	108.246, 209.027, 102.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.160 - 2.400
R-factor	0.2201
Rwork	0.218
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jln
RMSD bond length	0.007
RMSD bond angle	0.904
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.160	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.374
Number of reflections	45754	4436
<I/σ(I)>	11.57
Completeness [%]	99.4
Redundancy	12.7
CC(1/2)	0.998	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293	0.1 M Sodium Cacodylate pH 6.5, 0.45 M NaCl, 39% PEG400