7QLE

Crystal structure of S-layer protein SlpA from Lactobacillus acidophilus, domain I (aa 32-198)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97895
Spacegroup name	C 1 2 1
Unit cell lengths	130.127, 39.036, 60.058
Unit cell angles	90.00, 101.66, 90.00

Refinement procedure

Resolution	48.367 - 2.600
Rwork	0.200
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qld
RMSD bond length	0.008
RMSD bond angle	1.605
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.370	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.143	0.574
Rmeas	0.198	0.794
Rpim	0.137	0.547
Number of reflections	9254	893
<I/σ(I)>	7.1
Completeness [%]	99.3
Redundancy	3.3	3.4
CC(1/2)	0.986	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Protein stock solution of 15 mg/mL in 20 mM Hepes pH 8 and 100 mM NaCl; JCSG+ screen condition A5 (0.2 M Magnesium formate dihydrate, 20 % w/v PEG 3350) with protein end concentration of 7.5 mg/mL corresponding to 50% of protein solution in the 1.0 uL drop