7QIU

YsgA 23s RNA methyltransferase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980109
Spacegroup name	P 21 21 21
Unit cell lengths	64.880, 66.450, 129.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.849 - 1.877
R-factor	0.1833
Rwork	0.182
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kzk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.849	45.849	1.930
High resolution limit [Å]	1.877	8.400	1.880
Rmerge	0.049	0.023	1.255
Rmeas	0.050	0.024	1.298
Number of reflections	46483	589	3312
<I/σ(I)>	29.03	80.13	2.29
Completeness [%]	99.7	98.2	97.2
Redundancy	15.486	12.981	15.243
CC(1/2)	1.000	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	292	PEG3350 2%, MES 200mM pH 5.5