7QIP

SARS-CoV-2 Nucleocapsid phosphopeptide 201-210 bound to human 14-3-3 sigma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 41
Unit cell lengths	99.688, 99.688, 58.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.490 - 2.650
R-factor	0.2317
Rwork	0.230
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lu2
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.450	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	16912	818
<I/σ(I)>	4.8	0.9
Completeness [%]	100.0	100
Redundancy	11.9	13.1
CC(1/2)	0.989	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M AmSO4, 100 mM BisTris pH 5.5, 25% PEG 3350