7QIP
SARS-CoV-2 Nucleocapsid phosphopeptide 201-210 bound to human 14-3-3 sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 |
| Unit cell lengths | 99.688, 99.688, 58.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.490 - 2.650 |
| R-factor | 0.2317 |
| Rwork | 0.230 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lu2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.450 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 16912 | 818 |
| <I/σ(I)> | 4.8 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 13.1 |
| CC(1/2) | 0.989 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M AmSO4, 100 mM BisTris pH 5.5, 25% PEG 3350 |
