7QH5

The crystal structure of the sigma factor SigG1 from Streptomyces tsukubaensis NRRL18488

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	92.639, 151.836, 128.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.790 - 2.200
R-factor	0.2265
Rwork	0.225
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.860	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	46348	3984
<I/σ(I)>	13.1
Completeness [%]	99.9
Redundancy	13.8
CC(1/2)	0.999	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.8 M Sodium acetate trihydrate pH 7.0; 0.1 M BIS-TRIS propane pH 7.0