7QH3
Crystal structure of the anti-sigma factor RsfG from Streptomyces tsukubaensis NRRL18488
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.370, 61.130, 82.750 |
| Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
| Resolution | 70.270 - 2.300 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.270 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.087 | |
| Number of reflections | 31236 | 3091 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Ammonium phosphate dibasic; 0.1 M Tris hydrochloride pH 8.5; 0.5 M sodium phosphate dibasic dehydrate; 0.5 M potassium phosphate dibasic |
