7QFJ
Crystal structure of S-layer protein SlpX from Lactobacillus acidophilus, domain II (aa 194-362)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.94290 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.679, 143.016, 73.441 |
Unit cell angles | 90.00, 96.70, 90.00 |
Refinement procedure
Resolution | 46.028 - 2.500 |
Rwork | 0.206 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RoseTTAfold ab initio prediction |
RMSD bond length | 0.006 |
RMSD bond angle | 1.336 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.630 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.084 | 0.600 |
Rmeas | 0.100 | 0.715 |
Rpim | 0.053 | 0.384 |
Number of reflections | 46501 | 4625 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | 6.7 |
CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | Protein stock solution of 10 mg/mL in 20 mM Hepes pH 8 and 100 mM NaCl; Midas screen condition 50 (0.1M Tris, 50% v/v Pentaerythritol propoxylate (5/4 PO/OH)) with protein end concentration of 5 mg/mL corresponding to 50% of protein solution in the 1.0 uL drop, co-crystallization with 0.1 ul 2mM HgCl2 |