7QFG

Crystal structure of S-layer protein SlpA from Lactobacillus acidophilus, domain III (aa 309-444)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.25430
Spacegroup name	C 1 2 1
Unit cell lengths	100.628, 43.943, 42.488
Unit cell angles	90.00, 98.82, 90.00

Refinement procedure

Resolution	41.990 - 1.650
Rwork	0.159
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qeh
RMSD bond length	0.014
RMSD bond angle	1.908
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.990	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.074	0.150
Rmeas	0.102	0.206
Rpim	0.070	0.141
Number of reflections	22102	2193
<I/σ(I)>	11.3
Completeness [%]	99.4
Redundancy	3.2	3
CC(1/2)	0.985	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Protein stock solution of 20 mg/mL in 20 mM Hepes pH 8 and 100 mM NaCl; Index screen condition A9 (0.1 M BIS-TRIS pH 5.5, 3.0 M Sodium chloride) with protein end concentration of 10 mg/mL corresponding to 50% of protein solution in the 1.0 uL drop