7QFD

Crystal structure of a bacterial pyranose 2-oxidase complex with D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9654
Spacegroup name	C 2 2 21
Unit cell lengths	78.256, 78.489, 150.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.420 - 2.350
R-factor	0.2455
Rwork	0.245
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AsP2Ox WT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.110	2.460
High resolution limit [Å]	2.350	2.350
Number of reflections	18894	18815
<I/σ(I)>	4.11
Completeness [%]	96.3
Redundancy	5
CC(1/2)	0.970	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	2 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5