7QF3

Structure of the R57Q mutant of miniSOG expressed in E. coli in regular LB medium

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9322
Spacegroup name	P 43 21 2
Unit cell lengths	39.951, 39.951, 133.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.280 - 1.100
Rwork	0.144
R-free	0.17020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gpu
RMSD bond length	0.020
RMSD bond angle	2.246
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.280	1.140
High resolution limit [Å]	1.100	1.100
Rmeas	0.084	1.199
Number of reflections	45101	4488
<I/σ(I)>	15.5	1.9
Completeness [%]	100.0	100
Redundancy	12.4	11.9
CC(1/2)	1.000	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Tris-Hcl pH 8.0, 20 mM MgCl2, 28% PEG 4000, 15 mM CoCl2