Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-31 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 6 |
| Unit cell lengths | 92.360, 92.360, 57.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.656 - 2.304 |
| Rwork | 0.242 |
| R-free | 0.27750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xfx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.017 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.980 | 2.440 |
| High resolution limit [Å] | 2.304 | 2.304 |
| Rmeas | 0.071 | |
| Number of reflections | 12346 | 1951 |
| <I/σ(I)> | 10.76 | 1 |
| Completeness [%] | 98.4 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.997 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 9.5% PEG 3350 (v/v), 310 mM NaI, 100 mM Sodium Cacodylate. Crystals grew in 1 uL protein (3 mg/mL) + 1 uL crystallant. Cryoprotected in 20% (v/v) Glycerol. |
