7QCW
Apo-structure of serine hydroxymethyltransferase (PbzB) involved in benzobactin biosynthesis in P. chlororaphis subsp. piscium DSM 21509
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.400, 59.640, 112.120 |
| Unit cell angles | 90.00, 109.41, 90.00 |
Refinement procedure
| Resolution | 49.210 - 2.810 |
| R-factor | 0.2388 |
| Rwork | 0.236 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vmb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.210 | 2.910 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Number of reflections | 20309 | 1992 |
| <I/σ(I)> | 8.67 | |
| Completeness [%] | 99.6 | 98.47 |
| Redundancy | 4.9 | 5 |
| CC(1/2) | 0.994 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1M potassium chloride; 0.1 M HEPES pH7.5; 15% (w/v) PEG 6000 |
