7QCT

PDZ2 of LNX2 with SARS-CoV-2_E PBM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	I 4 3 2
Unit cell lengths	183.230, 183.230, 183.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 3.197
R-factor	0.2286
Rwork	0.228
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eiy
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	3.312
High resolution limit [Å]	3.197	3.197
Rmerge	0.268	3.061
Number of reflections	9009	861
<I/σ(I)>	20.81
Completeness [%]	99.7	97.95
Redundancy	78.7
CC(1/2)	0.999	0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	2.5 M NaCl, 0.1 M sodium acetate PH 4.5, 0.2 M lithium sulfate