7QCP

Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 7.2 - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96112
Spacegroup name	P 21 21 21
Unit cell lengths	71.278, 90.633, 95.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.650 - 1.110
R-factor	0.1426
Rwork	0.142
R-free	0.15640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7p5l
RMSD bond length	0.006
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.150
High resolution limit [Å]	1.110	1.110
Rmerge	0.066	1.116
Rmeas	0.069	1.178
Rpim	0.019	0.371
Number of reflections	242579	23553
<I/σ(I)>	16.7	2
Completeness [%]	99.9
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.2M Potassium Sodium Tartrate pH 7.2, 20% PEG 3350