7QBJ
bacterial IMPDH chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 4 |
| Unit cell lengths | 145.041, 145.041, 120.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.320 - 2.270 |
| R-factor | 0.197 |
| Rwork | 0.193 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dqw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.042 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 2.400 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.167 | |
| Rmeas | 0.173 | 0.891 |
| Number of reflections | 117473 | 18121 |
| <I/σ(I)> | 10.07 | |
| Completeness [%] | 99.7 | |
| Redundancy | 14 | |
| CC(1/2) | 0.993 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M TRIS 8.5 pH, 8 %w/v PEG 8K |
