7QBG

TC:CD320 in complex with nanobody TC-Nb4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979502
Spacegroup name	P 21 21 21
Unit cell lengths	105.339, 116.418, 125.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.920 - 2.690
R-factor	0.2299
Rwork	0.227
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrp
RMSD bond length	0.011
RMSD bond angle	2.025
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	2.790
High resolution limit [Å]	2.690	2.690
Number of reflections	84243	7177
<I/σ(I)>	7.97
Completeness [%]	98.0
Redundancy	2
CC(1/2)	0.993	0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	100 mM Bis-Tris pH 5.5, 25% w/v PEG 3350, supplemented with 10% (0.2% w/v Betaine anhydrous, 0.2% w/v L-Glutamic acid, 0.2% w/v L-Proline, 0.2% w/v Taurine, 0.2% w/v Trimethylamine N-oxide dihydrate, 0.02 M HEPES sodium pH 6.8