7QAZ

Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 - Primer Initiation Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	227.910, 39.620, 79.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.980 - 2.110
R-factor	0.2123
Rwork	0.210
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nqe
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	xia2 (3.4.2)
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.980	2.150
High resolution limit [Å]	2.110	2.110
Rmerge	0.151	1.042
Rmeas	0.174	1.382
Rpim	0.085	0.896
Number of reflections	40376	1427
<I/σ(I)>	6.5	0.6
Completeness [%]	94.4	69.6
Redundancy	3.8	1.9
CC(1/2)	0.992	0.298

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287.15	0.1M Sodium HEPES; MOPS (acid) 20% v/v Ethylene glycol; 10% w/v PEG 8000 0.03M Diethylene glycol; 0.03M Triethylene glycol; 0.03M Tetraethylene glycol; 0.03M Pentaethylene glycol 400uM DNA; 500uM AMPNPP; 4mM GTP; 2mM CoCl2; 130mM Monopotassium Glutamate