7Q82
Crystal structure of the methyltransferase-ribozyme 1, Thallium derivative (with 1-methyl-adenosine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9751 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.983, 71.983, 84.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.590 - 2.950 |
R-factor | 0.21 |
Rwork | 0.209 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7q7x |
RMSD bond length | 0.001 |
RMSD bond angle | 0.310 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.590 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.102 | |
Number of reflections | 5037 | 487 |
<I/σ(I)> | 18.01 | 0.81 |
Completeness [%] | 99.9 | 100 |
Redundancy | 24.9 | 26.7 |
CC(1/2) | 0.999 | 0.366 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 36-42% MPD, 50mM MES (pH 6.5), 10mM sodium acetate, 100mM lithium acetate, 10mM magnesium acetate, 25mM thallium acetate |