7Q7X

Crystal structure of the methyltransferase-ribozyme 1 (with 1-methyl-adenosine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9198
Spacegroup name	P 41 21 2
Unit cell lengths	71.037, 71.037, 82.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.940 - 2.800
R-factor	0.2147
Rwork	0.213
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7oax
RMSD bond length	0.001
RMSD bond angle	0.198
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.940	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.110
Number of reflections	5592	533
<I/σ(I)>	18.74	1.13
Completeness [%]	99.9	99.8
Redundancy	24.8	25.5
CC(1/2)	0.996	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	36-42% MPD, 50mM MES pH 6.4-6.7, 100mM NaCl, 100mM LiCl, 10mM MgCl2