7Q7Q

LIPIDIC CUBIC PHASE SERIAL FEMTOSECOND CRYSTALLOGRAPHY STRUCTURE OF A PHOTOSYNTHETIC REACTION CENTRE

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESA
Synchrotron site	SwissFEL ARAMIS
Beamline	ESA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-11-19
Detector	PSI JUNGFRAU 16M
Wavelength(s)	1.102
Spacegroup name	P 21 21 2
Unit cell lengths	84.660, 125.130, 182.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.702 - 2.250
Rwork	0.168
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nj4
RMSD bond length	0.008
RMSD bond angle	2.130
Data reduction software	CrystFEL (0.9.0)
Data scaling software	CrystFEL (0.9.0)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.702	2.270
High resolution limit [Å]	2.250	2.250
Number of reflections	92545	92545
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	11.888
CC(1/2)	0.995	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	This LCP crystallization was performed in wells with 200uL precipitant and 20uL LCP+protein.