7Q7O
Room temperature structure of the Rhodobacter Sphaeroides Photosynthetic Reaction Center F(M197)H mutant at atmospheric pressure after high helium gas pressure release
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2019-10-06 |
Detector | DECTRIS PILATUS3 X CdTe 2M |
Wavelength(s) | 0.477 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 102.520, 102.520, 237.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.070 - 2.650 |
R-factor | 0.1776 |
Rwork | 0.176 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v3y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.564 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13-2998_9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.070 | 2.745 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.475 | 3.133 |
Rmeas | 0.490 | 3.238 |
Rpim | 0.115 | 0.797 |
Number of reflections | 37727 | 3691 |
<I/σ(I)> | 6 | 0.96 |
Completeness [%] | 99.9 | 99.95 |
Redundancy | 17.1 | 15.5 |
CC(1/2) | 0.982 | 0.329 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 289 | 20-25 mg/ml protein solution was mixed 1:1 with lipid sponge phase. The mixture was filled into a sealed plastic tip covered with an excess amount of 1 M trisodium citrate and incubated in the dark at 289 K for 1 week. |