7Q7F

Room temperature structure of the Rhodobacter Sphaeroides Photosynthetic Reaction Center F(M197)H mutant at atmospheric pressure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	295
Detector technology	PIXEL
Collection date	2019-10-06
Detector	DECTRIS PILATUS3 X CdTe 2M
Wavelength(s)	0.477
Spacegroup name	P 42 21 2
Unit cell lengths	102.500, 102.500, 237.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.070 - 2.750
R-factor	0.1809
Rwork	0.179
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v3y
RMSD bond length	0.003
RMSD bond angle	0.555
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13-2998_9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.070	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.414	2.351
Rmeas	0.435	2.475
Rpim	0.128	0.751
Number of reflections	33826	3326
<I/σ(I)>	5.13	1.02
Completeness [%]	99.8	99.61
Redundancy	10.7	9.9
CC(1/2)	0.976	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		289	20-25 mg/ml protein solution was mixed 1:1 with lipid sponge phase. The mixture was filled into a sealed plastic tip covered with an excess amount of 1 M trisodium citrate and incubated in the dark at 289 K for 1 week.