7Q74

Structure of Pla1 apo, with a C-terminal deletion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 1
Unit cell lengths	71.790, 72.480, 73.210
Unit cell angles	99.96, 105.70, 118.83

Refinement procedure

Resolution	57.351 - 2.599
R-factor	0.2141
Rwork	0.212
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hhp
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.351	57.351	2.670
High resolution limit [Å]	2.599	11.620	2.600
Rmerge	0.150	0.037	1.439
Rmeas	0.178	0.045	1.707
Number of reflections	34690	389	2484
<I/σ(I)>	7.53	26.32	0.91
Completeness [%]	96.8	95.1	95
Redundancy	3.45	3.141	3.433
CC(1/2)	0.991	0.997	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	0.2 M sodium formate, 20% PEG 3350