7Q6I

Vibrio maritimus FtsA 1-396 ATP and FtsN 1-29, bent tetramers in double filament arrangement

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.00915
Spacegroup name	P 1 21 1
Unit cell lengths	98.692, 119.993, 329.788
Unit cell angles	90.00, 93.76, 90.00

Refinement procedure

Resolution	49.880 - 3.600
R-factor	0.2428
Rwork	0.241
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7q6f
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.980	75.980	3.690
High resolution limit [Å]	3.600	16.100	3.600
Rmerge	0.163	0.049	1.197
Rmeas	0.177	0.053	1.294
Rpim	0.067	0.020	0.489
Total number of observations	620636	7006	46750
Number of reflections	89730	1072	6579
<I/σ(I)>	8.6	23	1.8
Completeness [%]	100.0	98.9	100
Redundancy	6.9	6.5	7.1
CC(1/2)	0.997	0.997	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	21.645 % v/v PEG 600, 0.125 M MgCl2, 0.1 M Tris/HCl pH 8.5