7Q6G

Xenorhabdus poinarii FtsA 1-396 ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.00915
Spacegroup name	C 1 2 1
Unit cell lengths	142.276, 70.715, 50.719
Unit cell angles	90.00, 100.98, 90.00

Refinement procedure

Resolution	40.830 - 2.800
R-factor	0.2201
Rwork	0.218
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7q6d
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.850	40.850	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.257	0.119	0.857
Rmeas	0.306	0.142	1.041
Rpim	0.165	0.077	0.584
Total number of observations	41563	463	2652
Number of reflections	12283	141	895
<I/σ(I)>	4.4	9	1.1
Completeness [%]	99.9	97.6	99.9
Redundancy	3.4	3.3	3
CC(1/2)	0.796	0.952	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	1 M sodium succinate pH 7.0, 0.1 M bis-tris-propane pH 7.0