7Q6F

Vibrio maritimus FtsA 1-396 ATP, double filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	48.503, 109.628, 92.490
Unit cell angles	90.00, 102.14, 90.00

Refinement procedure

Resolution	47.420 - 3.310
R-factor	0.2322
Rwork	0.230
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7q6d
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	109.760	109.760	3.400
High resolution limit [Å]	3.310	14.800	3.310
Rmerge	0.297	0.086	1.309
Rmeas	0.321	0.092	1.419
Rpim	0.120	0.034	0.544
Total number of observations	98562	1172	6686
Number of reflections	14187	177	1038
<I/σ(I)>	5.9	9.7	2.2
Completeness [%]	99.4	99.7	99
Redundancy	6.9	6.6	6.4
CC(1/2)	0.978	0.995	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	25.236 % v/v PEG 400, 2 % v/v 2 propanol, 0.17 M MgCl2, 0.04 M CaAc2, 0.08 M Tris/HCl pH 8.5, 0.02 M MES/NaOH pH 6.0