7Q6B
mRubyFT/S148I, a mutant of blue-to-red fluorescent timer in its blue state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.79312 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.790, 66.828, 97.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.880 - 1.800 |
| R-factor | 0.2291 |
| Rwork | 0.227 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u0l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.103 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.880 | 48.880 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.080 | 0.027 | 1.200 |
| Rmeas | 0.090 | 0.031 | 1.334 |
| Rpim | 0.039 | 0.015 | 0.576 |
| Total number of observations | 96802 | 667 | 5805 |
| Number of reflections | 19640 | 171 | 1116 |
| <I/σ(I)> | 6.1 | 54.1 | 0.2 |
| Completeness [%] | 98.2 | 86.5 | 96.7 |
| Redundancy | 4.9 | 3.9 | 5.2 |
| CC(1/2) | 0.998 | 0.998 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 0.1M Bis-tris pH 5.5; 19% PEG 3350 |
