7Q5D
Structure of EPCR in a non-canonical conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979182 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.470, 70.470, 96.635 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.880 - 1.800 |
| R-factor | 0.1841 |
| Rwork | 0.183 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l8j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.168 |
| Data reduction software | XDS (BUILT=20200131) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.640 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 1.004 |
| Rmeas | 0.036 | 1.101 |
| Rpim | 0.015 | 0.447 |
| Number of reflections | 26315 | 1531 |
| <I/σ(I)> | 21.1 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6 |
| CC(1/2) | 0.999 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium-potassium tartrate, 20% w/v PEG 3350 |
