7Q51

yeast Gid10 bound to a Phe/N-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	99.228, 99.228, 81.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.970 - 2.220
R-factor	0.1888
Rwork	0.187
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ns3
RMSD bond length	0.004
RMSD bond angle	0.795
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.970	2.350
High resolution limit [Å]	2.220	2.220
Number of reflections	22659	6117
<I/σ(I)>	22.54
Completeness [%]	97.6
Redundancy	13
CC(1/2)	1.000	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	0.1 M MES pH 6.9