7Q4G
Structure of coproheme decarboxylase from Corynebacterium dipththeriae Y135A mutant in complex with coproheme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.117, 123.517, 78.300 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 48.310 - 1.820 |
| R-factor | 0.1739 |
| Rwork | 0.173 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xuc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4380) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.310 | 1.885 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.191 | |
| Rmeas | 0.207 | 2.079 |
| Rpim | 0.080 | 0.943 |
| Number of reflections | 659722 | 8371 |
| <I/σ(I)> | 5.73 | |
| Completeness [%] | 97.7 | 81.68 |
| Redundancy | 6.6 | 4.6 |
| CC(1/2) | 0.995 | 0.221 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | PEG 3350, MgCl2, Sodium Phosphate |
