7Q46

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of pericentriolar material 1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000292690818826
Spacegroup name	P 43 21 2
Unit cell lengths	107.660, 107.660, 241.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.156 - 2.460
R-factor	0.181991702573
Rwork	0.179
R-free	0.24534
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.007
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.156	2.520
High resolution limit [Å]	2.460	2.460
Number of reflections	52438	3788
<I/σ(I)>	6.33	1.2
Completeness [%]	100.0
Redundancy	26.5269079675
CC(1/2)	0.984	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	hanging drop 20% PEG 3350 0.18M tris amonium citrate