7Q40

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978565
Spacegroup name	P 43 21 2
Unit cell lengths	108.540, 108.540, 243.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.270 - 2.350
R-factor	0.181466050477
Rwork	0.179
R-free	0.23032
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.007
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.270	2.410
High resolution limit [Å]	2.350	2.350
Number of reflections	60722	4062
<I/σ(I)>	13.21
Completeness [%]	98.8
Redundancy	26.6657389414
CC(1/2)	0.998	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	hanging drop 20% PEG 3350 0.18M tris amonium citrate