7Q3V
Re-refined structure of a type III antifreeze protein isoform HPLC 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.270, 39.890, 44.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.860 - 1.900 |
| R-factor | 0.1632 |
| Rwork | 0.160 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1msi |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | AMBER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.860 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19230 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50-55% AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4-4.5 |
