7Q3S

Crystal structure of UGT706F8 from Zea mays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	50.188, 64.874, 76.502
Unit cell angles	90.00, 93.79, 90.00

Refinement procedure

Resolution	43.200 - 1.590
R-factor	0.1678
Rwork	0.167
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nlm
RMSD bond length	0.017
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.062	1.001
Rpim	0.026	0.424
Number of reflections	62014	4725
<I/σ(I)>	17.56
Completeness [%]	94.9	72.97
Redundancy	6.9	1.4
CC(1/2)	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1	293	PEG 6000 (20%), Tris 100mM, NaCl 200mM, pH 8.1 12 mg/ml 2:1 drops