7Q3O
Structure of CDX1 bound to hydroxymethylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972420 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.373, 64.669, 67.028 |
| Unit cell angles | 112.37, 108.11, 93.93 |
Refinement procedure
| Resolution | 42.180 - 2.780 |
| R-factor | 0.2203 |
| Rwork | 0.217 |
| R-free | 0.28570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lux |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.734 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.180 | 42.180 | 2.930 |
| High resolution limit [Å] | 2.780 | 8.780 | 2.780 |
| Rmerge | 0.058 | 0.026 | 0.693 |
| Rmeas | 0.078 | 0.036 | 0.944 |
| Rpim | 0.052 | 0.024 | 0.638 |
| Total number of observations | 30774 | 898 | 4282 |
| Number of reflections | 14867 | 451 | 2121 |
| <I/σ(I)> | 7.6 | 18.5 | 1 |
| Completeness [%] | 90.0 | 85.7 | 87.6 |
| Redundancy | 2.1 | 2 | 2 |
| CC(1/2) | 0.997 | 0.997 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 3350, potassium chloride, magnesium chloride, PEG 200, Tris |
