7Q3C
Ribonucleotide Reductase AaR2 protein from Aquifex aeolicus
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.183, 69.183, 177.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.968 - 2.150 |
Rwork | 0.247 |
R-free | 0.31450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ail |
RMSD bond length | 0.003 |
RMSD bond angle | 1.194 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.968 | 2.220 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 24392 | 2058 |
<I/σ(I)> | 24.6 | |
Completeness [%] | 100.0 | |
Redundancy | 25.1 | |
CC(1/2) | 1.000 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M citrate pH 4.0, 1.0 M lithium chloride, 20 % PEG6000 |