7Q37
Crystal structure of proton pump MAR rhodopsin pressurized with krypton
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.861 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 50.897, 40.381, 60.619 |
| Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
| Resolution | 59.430 - 2.250 |
| R-factor | 0.18117 |
| Rwork | 0.178 |
| R-free | 0.24468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jsi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmeas | 0.140 | 1.299 |
| Number of reflections | 22357 | 2971 |
| <I/σ(I)> | 7.74 | 1.14 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.996 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 2.6 M ammonium sulfate, 0.1 M sodium acetate, pH 5.2 |
