7Q30
Mutant T91A of uridine phosphorylase from Shewanella oneidensis
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | KURCHATOV SNC BEAMLINE K4.4 |
Synchrotron site | KURCHATOV SNC |
Beamline | K4.4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-27 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.964 |
Spacegroup name | P 3 |
Unit cell lengths | 91.660, 91.660, 47.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.474 - 1.901 |
Rwork | 0.157 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r2x |
RMSD bond length | 0.016 |
RMSD bond angle | 2.425 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 35104 | 5812 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 99.4 | |
Redundancy | 6.38 | |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 8000, 1 M lithium sulfate monohydrate |