7Q1J
Hybrid form of uridine phosphorylase from E. coli and Salmonella typhimurium in the presence PEG
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8025 |
| Spacegroup name | H 3 |
| Unit cell lengths | 150.590, 150.590, 46.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.710 |
| Rwork | 0.204 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.609 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Number of reflections | 42058 | 3091 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.82 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Potassium citrate tribasic monohydrate, 20% w/v Polyethylene glycol 3,350 |
