7Q1F

CPAP:TUBULIN:IE5 ALPHAREP COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	89.088, 215.864, 95.536
Unit cell angles	90.00, 109.61, 90.00

Refinement procedure

Resolution	107.930 - 2.347
R-factor	0.1897
Rwork	0.188
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gwc
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.932	2.609
High resolution limit [Å]	2.347	2.347
Rmeas	0.189	1.107
Rpim	0.070	0.415
Number of reflections	97303	4866
<I/σ(I)>	7.1	1.6
Completeness [%]	90.8
Redundancy	7.2
CC(1/2)	0.994	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.8	293	0.18 M tri-ammonium citrate, 0.1 M Mes-K pH 6.8, 18.5 % PEG3350