7Q0V

Lysozyme soaked with V(IV)OSO4 and phen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	77.680, 77.680, 37.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.840 - 1.120
R-factor	0.14877
Rwork	0.147
R-free	0.17557
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.011
RMSD bond angle	1.549
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.840	1.140
High resolution limit [Å]	1.120	1.120
Number of reflections	44798	2077
<I/σ(I)>	21.3	2.2
Completeness [%]	99.6
Redundancy	9.9
CC(1/2)	1.000	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	4-8% (M/V) NACL IN 0.1 M ACETATE BUFFER PH 4.5. PROTEIN STOCK CONCENTRATION OF 50 MG/ML.