7Q0T

Lysozyme soaked with V(IV)OSO4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9797
Spacegroup name	P 43 21 2
Unit cell lengths	78.102, 78.102, 36.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.050 - 1.340
R-factor	0.12883
Rwork	0.127
R-free	0.17066
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.018
RMSD bond angle	1.829
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.050	1.360
High resolution limit [Å]	1.340	1.340
Number of reflections	26000	1272
<I/σ(I)>	18.4	2.1
Completeness [%]	99.9
Redundancy	7.4
CC(1/2)	0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	4-8% (M/V) NACL IN 0.1 M ACETATE BUFFER PH 4.5. PROTEIN STOCK CONCENTRATION OF 50 MG/ML.