7Q0M

Crystal structure of the peptide transporter YePEPT-K314A in complex with LZNV at 2.66 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	88.398, 100.372, 103.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.960 - 2.540
R-factor	0.2683
Rwork	0.267
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7q0l
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.850	2.748	2.660
High resolution limit [Å]	2.540	2.657	2.540
Number of reflections	24643	1141	1158
<I/σ(I)>	19.7	1.37	1.2
Completeness [%]	95.1	81.9	79.3
Redundancy	10.9	11.7	11.5
CC(1/2)	0.998	0.579	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.25	291	PEG 300, glycine, LZNV