7Q0L

Crystal structure of the peptide transporter YePEPT-K314A at 2.93 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	89.981, 101.064, 103.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.814 - 2.710
R-factor	0.262
Rwork	0.260
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w6v
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.124	2.949	2.842
High resolution limit [Å]	2.710	2.842	2.710
Number of reflections	21272	1063	1065
<I/σ(I)>	14.9	1.562	1.169
Completeness [%]	92.8	71	75.1
Redundancy	5.7	5.8	5.7
CC(1/2)	1.000	0.523	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	291	PEG 300, NaOAc, LiH2PO4, Li2SO4